Composite

Part:BBa_K1321299:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-20)


J23101-GFP in pSEVA321-Bb


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4499
    Illegal suffix found in sequence at 928
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4499
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 929
    Illegal PstI site found at 943
    Illegal NotI site found at 936
    Illegal NotI site found at 4505
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4499
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4499
    Illegal suffix found in sequence at 929
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4499
    Illegal XbaI site found at 4514
    Illegal SpeI site found at 929
    Illegal PstI site found at 943
    Illegal NgoMIV site found at 2118
    Illegal NgoMIV site found at 3003
    Illegal NgoMIV site found at 4114
    Illegal NgoMIV site found at 4238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


Design Notes

BBa_K1321330 was created by restricting BBa_K1321301 and BBa_I20260 with PstI and XbaI, gel purifying the resulting fragments, ligating using T4 DNA ligase and transforming the ligation reaction into E.coli DH10B.

Source

This is a composite part of BBa_I20260 and BBa_K1321300

References